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BIOCHEMICAL PROPERTIES OF BLOOD IN BLACK-LAKE LAMBS
INFECTED WITH ECHINOCOCCOSIS IN THE DESERT CONDITIONS OF
UZBEKISTAN
Azzamov U.A.
Zarmed University Medical Biology, Biophysics, Microbiology
and instructor of the Department of IT in Medicine
Artikova Sh.A
Therapeutic Work at Zarmed University
Student of group 145
Currently, scientific research on the sustainable development of animal
husbandry, increasing the resistance of animals to diseases, and ensuring their
productivity is of particular importance. In sheep breeding, which is an important
branch of animal husbandry in the steppe region of Uzbekistan, parasitic diseases
such as echinococcosis have a negative impact on the health and productivity of
fungi. The topic of this study is devoted to the analysis of biochemical changes in the
blood of Black Lake lambs in steppe conditions and the study of the impact of
echinococcosis on their health. On a global scale, the protection of animals from
diseases is of great importance in ensuring their productivity and health. In particular,
in order to increase the production of high-quality meat, dairy, and wool products in
the livestock sector of the Republic of Uzbekistan, large-scale scientific research is
being conducted to combat animal diseases and maintain their health. Echinococcosis
is currently recognized as one of the factors reducing economic efficiency and
negatively affecting the overall health and development of fungi.
Echinococcosis is a disease caused by the infection of animals with the larvae of
helminths of the genus Echinococcus granulosus, which poses a danger not only to
sheep and goats, but also to humans. The prevalence and consequences of this disease
are a pressing scientific and practical problem, especially in the lambs of the Black
Lake in the steppe region. For steppe mushrooms, specific climatic and
environmental conditions affect their health and immunity, which is important in the
development of the disease.
The purpose of this study is to determine the biochemical changes in the blood
of Black Lake lambs in the steppe region and to assess the influence of
echinococcosis on them. The results in this regard will help not only improve animal
health but also reduce economic losses.
At the same time, the results of this study make it possible to develop
scientifically based preventive and therapeutic measures. The length of the mature
ribbon-like form of echinococcus is about 2-6 mm. The finnoz period of
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echinococcus, on the contrary, is the size of an apple, a child's head, sometimes
extremely large (up to 16-20 kg). The echinococcal div, like in other tapeworms,
consists of scolex, immature, hermaphrodite, and mature proglottids. The scolex of
echinococcus has four adhesive devices, a proboscis, and two rows of hooks located
on the proboscis. After the scolex, there are a total of 3 or 4 proglottids, the anterior
parts of which are immature, and the posterior parts are mature. The last, mature
proglottid is longer than other proglottids, with a width of 0.5-0.6 mm. Mature
proglottids have a short, branched uterus with fertilized eggs. This type of uterine
structure is important in diagnostics. The ribbon-shaped organs of echinococcus -
digestive, excretory, respiratory organs, and nervous system - are structured similarly
to the bull's tapeworm. The mature proglottid of echinococcus with fertilized eggs is
excreted with feces from the small intestine of the main host - dogs, wolves, and
foxes. In the external environment, as a result of entering water, grass, and soil, it
enters cattle, horses, pigs, and other animals, as well as humans. The area around the
anus of the main hosts becomes dirty, and their wool becomes dirty as a result of
lying on dirty ground and falling. Consequently, hunting these animals, skinning
them, and dissecting their bodies increases the risk of contracting the disease. Mature
echinococcus eggs are transferred to intermediate hosts - humans, ungulates, and
cattle - through the above methods or through the consumption of water and herbs,
where they undergo the Finnoz period. Humans often contract this disease through
contact with dogs (touching them, bringing them close to household items, etc.).
Therefore, young children are more susceptible to this disease than adults.
By studying the biochemical changes in the blood of Black Lake lambs, it
becomes possible to determine the methods of diagnosis and prevention of the
disease. Also, the obtained results are of great scientific and practical importance, as
they provide new scientific knowledge in the fields of parasitology and biochemistry.
Therefore, the tasks set in this dissertation work are to determine the biochemical
parameters of the blood of black lake lambs, assess the negative impact of
echinococcosis, and improve diagnostic methods.
Echinococcosis is a parasitic disease caused by echinococcal species that causes
serious economic and health problems for livestock and humans. Studying the degree
of echinococcosis infection and the resulting metabolic changes in the desert
conditions of Uzbekistan is one of the urgent tasks. The purpose of this study is to
analyze the biochemical parameters of the blood of Karakul lambs under the
influence of echinococcosis.
Materials and methods
: In the study, lambs from Kara-Kul were selected as a
sample. The experimental group consisted of lambs infected with echinococcosis, and
the control group consisted of healthy lambs. In blood samples, the levels of total
protein, albumin, globulins, aminotransferases (ALT and AST), bilirubin, and
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glucose were analyzed. These biochemical indicators were measured using special
reagents using laboratory methods. A venous blood sample was taken from each
lamb. The following parameters were analyzed in blood samples:
1. Total protein and its fractions (albumin, globulin):
These indicators reflect
the overall metabolic activity of the div and the state of the immune system.
2. Aminotransferases
(AST and ALT): are the main indicators of liver
function.
3. Bilirubin:
This plays an important role in assessing liver and hemolytic
processes.
4. Sugar content:
Indicates the state of sugar metabolism as the main source of
energy.
Laboratory analyses were conducted using a special biochemical analyzer. Each
parameter was measured on average 3-5 times and analyzed based on statistical
reliability criteria. Results and discussion
The results showed:
- Levels of total protein and albumin: In lambs infected with echinococcosis,
there was a significant decrease compared to the control group. These proteins
decrease due to infection with parasites, as the immune response and inflammatory
processes in the div are activated.
Aminotransferases: An increase in AST and ALT indicates the presence of
inflammatory or necrotic processes in the liver.
- Bilirubin: An increase in the level of bilirubin in affected lambs confirms the
activation of hemolytic processes and impaired liver function.
- Sugar levels: Changes in sugar levels have been observed as a result of
parasitic infection, indicating disruptions in the div's energy metabolism.
One of the important results is that in lambs infected with echinococcosis, the
general metabolic activity of the div is disrupted. This leads to reduced productivity
and increased susceptibility to other diseases.
Results and discussion: The results of the study showed that in lambs infected
with echinococcosis, the levels of total protein and albumin in the blood were
significantly reduced compared to the control group. This indicates a disruption of the
div's overall metabolic activity as a result of a parasitic infection. An increase in
aminotransferase indicators confirms a decrease in liver function. In addition,
elevated bilirubin levels indicate hemolysis and impaired liver function.
N
o
Parameter
Function
Changes
in
Infected Lambs
1
Total protein
and fractions
Reflects the div's
metabolic
activity
and
Significantly
decreased. Indicates
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immune status.
immune
response
and
inflammation
due
to
infection.(albumin,
globulin)
2
Aminotransfe
rases (AST and
ALT)
Key indicators of liver
function.
Elevated levels
indicate
inflammation
or
necrotic processes in
the liver.
3
Bilirubin
Important for assessing
liver
and
hemolytic
processes.
Increased levels
confirm
impaired
liver function and
active
hemolytic
processes.
4
Glucose level
Indicates the state of
carbohydrate metabolism as
the main energy source.
Fluctuations
observed
due
to
parasite
infection,
indicating
energy
metabolism
disturbances.
Conclusion
Biochemical changes in the blood of Karakul lambs infected with
echinococcosis in the desert conditions of Uzbekistan were determined. These
changes reflect metabolic stress resulting from parasitic infection. Based on the
research results, it is recommended to develop measures for the prevention and
treatment of echinococcosis.
References:
1.
WHO. Echinococcosis Fact Sheet. World Health Organization, 2023.
2.
Tursunov A., Echinococcosis in Animals. Veterinary Journal, 2022.
3.
O‘zbekiston qishloq xo‘jaligi vazirligi ma’lumotlari, 2024.
4.
Azimovich AUB, G'iyosovna SD, Zokirovna MM XLAMIDIYANING
INSON SALOMATLIGIGA TA'SIRINI MIKROBIOLOGIK TAHLILLI VA
DIOGNOSTIKASI //Talqin va tadqiqot ilmiy-uslubiy jurnali. – 2022. – T. 1. –
№. 11. – S. 153-161.
5.
Azimovich A.U., Sultonuvich B.K., Zokirovna MM STREPTOKOKK
AVLODIGA
MANSUB
BAKTERIYALARNING
PATOGENLIK
International scientific journal
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Volume 1 issue 11 (57) | ISSN: 2181-4163 | Impact Factor: 8.2
139
XUSIYATLARINING TAHLILI //Talqin va tadqiqot ilmiy-uslubiy jurnali. – 2022. –
T. 1. – №. 13. – S. 95-101.
6.
Azzamov Ulug‘Bek Azimovich, Shodiyeva Dildora G‘Iyosovna,
Maxmudov Aziz Akmalovich ANTIBIOTIKLAR TA'SIR DOIRASIGA KO'RA
KLASSIFIKATSIYASI // Talqin va tadqiqotlar ilmiy-uslubiy jurnali. 2023. №17.
URL:
https://cyberleninka.ru/article/n/antibiotiklar-tasir-doirasiga-kora-
klassifikatsiyasi (дата обращения: 28.04.2023).
7.
Azzamov,
U.,
&
Yorbekova,
S.
(2023).
AYRIM
ANTIBIOTIKLARNING FARMAKOLOGIK TA’SIRINI MIKROBIOLOGIK
SOLISHTIRMA TAHLILI.
Interpretation and Researches
,
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(6). извлечено от
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