INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE
ISSN: 2692-5206, Impact Factor: 12,23
American Academic publishers, volume 05, issue 07,2025
Journal:
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UDK: 617:576.8:615
MODERN AND EFFECTIVE APPROACHES TO THE TREATMENT OF
CANINE WOUNDS
Narziyev Bakhtiyor Daliyevich
Scientific Supervisor, Professor.
Yuldasheva Madina Kakhramonovna
Doctoral Student.,
Ravshanov Mirjalol Akmalovich
,
asistent, PhD.Samarkand State University of Veterinary Medicine, Livestock
and Biotechnologies, Samarkand, Uzbekistan
Annotation.
This article presents the results of a study on the treatment of purulent wounds in
dogs using the probiotic Innoprovet2 in various combinations (with and without antibiotic). It
was found that under the influence of Innoprovet2 the average period of wound healing in dogs
was: in the first experimental group 22-24 days, in the second experimental group 16-18 days,
and in the control group - 26-28 days. During the experiment, the wound healing process and
the condition of the dogs were observed for 28 days. ImitoMeasure program was used to
evaluate the wound surface in all groups, and a systematic evaluation of the wound condition
was performed.
Keywords:
Wound, probiotic, ImitoMeasure, antibiotic, ciprofloxacin, pus, sorbents, infection.
Introduction
At present, in both human and veterinary medicine, surgical disorders
account for approximately 30–35% of cases, frequently manifesting as acute and chronic
purulent diseases. The lack of comprehensive treatment strategies for wound processes disrupts
homeostasis and impairs the function of internal organs, which in turn diminishes the efficacy
of pharmacological interventions and contributes to the development of complications [3].
A wide range of therapeutic agents—including antibiotics, proteolytic enzymes,
bioactive sorbents, and physical treatment modalities such as laser therapy, ultrasound, and
hyperbaric oxygenation—are routinely employed in the management of wounds and wound-
related infections [2]. However, due to the limited scope of these therapeutic approaches, the
treatment of purulent wounds remains a pressing challenge. Therefore, the development of
novel therapeutic strategies and the implementation of environmentally safe agents constitute a
major focus of contemporary research efforts [5,7,8,9].
Probiotic preparations can be administered in various formulations, including powders,
pastes, or aerosols, often combined with auxiliary substances or sorbent mixtures [5]. A
significant proportion of pathogenic microorganisms implicated in wound infections exhibit
resistance to conventional antibiotics. Among the promising candidates with both antimicrobial
and regenerative properties is the probiotic bacterium Bacillus subtilis, which is capable of
synthesizing antibiotics [1,4] and bacteriocins [5].
These bacteria also enhance wound healing by producing proteolytic enzymes,
demonstrating antioxidant activity [1,4,5,6], exerting thrombolytic effects, and preventing
excessive scar formation. Several authors have reported the successful application of probiotic
INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE
ISSN: 2692-5206, Impact Factor: 12,23
American Academic publishers, volume 05, issue 07,2025
Journal:
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formulations such as Bioseptin, Bactisporin, Sporobacterin, and Bactisporin for the treatment of
uninfected wounds, purulent-necrotic processes, burns, and dermatitis.
Objective of the Study
This research was dedicated to investigating the effects of
probiotic administration in the treatment of purulent wounds in dogs.
Object of the Study
Nine dogs were selected as experimental subjects and randomly
allocated into three groups: two experimental groups and one control group. In the experimental
animals, standardized wounds were created in the femoral region using the method described by
Sukhovei (2008).
Study Site
The experiments were conducted at the Veterinary Surgery and Obstetrics
Department Clinic of Samarkand State University of Veterinary Medicine, Animal Husbandry
and Biotechnologies.
Materials and Methods
To assess the therapeutic properties of the probiotic Inoprovet-
2, the nine dogs were divided into three groups. In all animals, wounds were surgically induced
on the lateral surface of the thigh and inoculated with Streptococcus pyogenes isolated in
previous studies to establish a model of purulent infection.
After 72 hours, upon confirmation of purulence, all groups received daily wound
irrigation with a 1:5000 furacilin solution.
In Experimental Group I (three dogs), drainage with Inoprovet-2 probiotic was applied
to the wounds, and the probiotic was also administered orally at a dose of 5 ml of suspension
(containing 1×10⁹ CFU/ml) twice daily.
In Experimental Group II, the probiotic was given orally at the same dosage, and
ciprofloxacin was administered intramuscularly at a dose of 1 ml.
In the Control Group (Group III), wounds were treated topically with 0.4 ml of
ciprofloxacin solution.
For oral administration, the dry probiotic was reconstituted at a concentration of 1 g per
1,000 ml of water. For topical application (drainage), it was diluted at a ratio of 1 g per 100 ml
of physiological saline (tab-1).
Table 1. Experimental design for assessing the efficacy of Inoprovet-2
probiotic in dogs with purulent wounds
Group
Number
of Dogs
Treatment Modality
Dosage and Application Details
Experimental
Group I
3
Inoprovet-2
probiotic
(oral + topical drainage)
Oral: 5 ml of suspension (1×10⁹
CFU/ml) twice daily; Topical:
drainage application diluted 1 g in
100 ml saline
Experimental
Group II
3
Inoprovet-2
probiotic
(oral) + ciprofloxacin
(intramuscular)
Oral
probiotic
as
above;
Intramuscular ciprofloxacin: 1 ml
once daily
Control Group
III
3
Ciprofloxacin (topical)
Topical application: 0.4 ml solution
to wound surface
Results of Clinical and Planimetric Evaluation of Purulent Wound Healing in Dogs
According to the results of clinical and planimetric observations, during the initial days
following the induction of purulent wounds, all three groups of dogs exhibited marked systemic
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reactions. General malaise was noted, with div temperature rising by 1–1.5 °C (reaching 39–
40 °C). Heart rate increased up to 120 beats per minute, and respiratory rate reached 37 breaths
per minute.
On palpation, wounds were painful and edematous, with irregular and rounded edges
covered by serous exudate. The wound surfaces were moist and shiny, with mild swelling and
uniform hyperemia observed across all groups.
By day 7, a slight reduction in wound area was recorded in both the control group and
the first experimental group. In the control group, the wound surfaces appeared dark red, moist,
and with moderately irregular margins. Palpation elicited pain, and purulent discharge was
present. In contrast, dogs in the second experimental group developed dry, thin black scabs
covering the wound surface.
On day 14, granulation tissue proliferation was evident in the control and first
experimental groups. The wound periphery remained mildly edematous but was no longer
painful. In the control animals, the black scab persisted along the wound surface, which
remained slightly moist. In the second experimental group, the wound margins appeared
smooth, with no pain upon palpation. Visual examination showed clear reduction in wound size,
and scar formation along the edges was observed, indicating the onset of epithelialization.
Throughout the study period, clinical indicators and general condition of all dogs were assessed
as satisfactory, with preserved appetite in all groups. Quantitative wound measurements were
performed in all animals using the ImitoMeasure smartphone application for digital analysis.
On day 21, in the control group, a uniform black scab covered the entire wound surface,
with smooth and rounded wound margins.
In the first experimental group, progressive epithelialization was observed along the
wound periphery. In the second experimental group, complete epithelialization had occurred,
accompanied by visible hair regrowth within the wound area. Wound healing and the overall
condition of the animals were monitored for a total of 28 days.
The mean duration required for complete wound closure varied between groups:
In the control group, complete healing occurred between days 26 and 28.
In the first experimental group, wound closure was achieved between days 22 and 24.
In the second experimental group, healing was completed significantly earlier, between
days 16 and 18.
Following the treatment interventions, the reduction in wound area on day 21
demonstrated marked intergroup differences:
Table 2. Healing Time and Residual Wound Area in Experimental Groups
Group
Time to Complete Healing
(days)
Residual Wound Area on Day 21
(cm²)
Control Group
26–28 (mean: 27 ± 0.8)
1.0 ± 0.4
Experimental Group I
22–24 (mean: 23 ± 0.7)
0.7 ± 0.2
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Experimental Group II
16–18 (mean: 17 ± 0.6)
0.3 ± 0.1
Healing Time: F(2,6)=52.3, p < 0.001
Residual Wound Area: F(2,6)=28.6, p < 0.001
In the control group (treated with furacilin irrigation and topical ciprofloxacin), the
average residual wound area measured 1.0 ± 0.4 cm².
In the first experimental group (furacilin irrigation combined with oral probiotic
administration and probiotic drainage), the residual wound area was 0.7 ± 0.2 cm². In the second
experimental group (furacilin irrigation, oral probiotic administration, topical probiotic
application, and intramuscular ciprofloxacin), complete wound closure was observed in the
majority of animals, with minimal residual wound area averaging 0.3 ± 0.1 cm².
Conclusion
The results of this study demonstrated that the treatment regimen
combining oral administration of Inoprovet-2 probiotic, initial wound irrigation with furacilin
solution, subsequent probiotic drainage, and intramuscular ciprofloxacin effectively promoted
debridement of necrotic tissues and reduced edema within 4–5 days. This approach also
stimulated granulation tissue formation and significantly accelerated the overall wound healing
process compared to conventional treatment methods.
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ISSN: 2692-5206, Impact Factor: 12,23
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Journal:
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