399
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF BIOACTIVE SUBSTANCES IN OIL EXTRACT
Mirzalieva G.
Pulatova F.A.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: pulatovaf652@gmail.com, tel: (99)3006128
https://doi.org/10.5281/zenodo.17340297
Relevance:
Vitamin E is a group of important natural compounds, the basis of which is
tocopherols and tocotrienols. Tocopherols are used as antioxidants, the most powerful antioxidant is
δ-tocopherols. They have a strong neuroprotective, antioxidant effect, and also reduce the risk of
cancer. In addition, they have an antihypoxant effect, reducing the need for oxygen in cells.
It protects cells from damage by free radicals, thereby slowing down the aging process. All
compounds in this group are fat-soluble.
Vitamin E is important in dermatological practice as it is used as the main component in the
elimination of various problems and in the production of cosmetics.
It improves skin nutrition, increases its elasticity and firmness, helps in wound healing and
reduces dryness. It is involved in energy metabolism and facilitates the absorption of other vitamins,
such as vitamin A.
Purpose of the study:
The purpose of this study is to determine the amount of vitamin E in the
oil extracts (ME-1 and ME-2) obtained in two different ways from the local medicinal plants: Calami
rhizomata, Herba hyperici, Radices Glicyrrhizae, Herba Bidentis and fruit Fructus Rosae.
Methods and techniques:
Based on the purpose of the study, an oil extract was obtained from
a mixture of the above-mentioned plants in a certain ratio in two ways: in method 1, it was extracted
using a simple hot maceration method and in method 2, it was extracted using sunflower oil as an
extractant using ultrasound. Taking into account the use of the obtained ME in dermatology practice,
liquid chromatography was used to determine the amount of bioactive substance vitamin E in its
content. Chromatography was performed on a liquid chromatograph of the Agilent Technologies
(USA) brand "Agilent 1260 series" with the "Chemstation 09.03.a" software, gradient pump and
spectrophotometric detector. Separation was performed on an Agilent-1200-5 micron C18 column
with an internal diameter of 4.6 mm, a length of 150 mm, and a sorbent particle size of 5 μm or
similar. Dichloromethane (A) and acetonitrile (B) (in a volume ratio of 15:85) were used as the mobile
phase. Detection was performed at a wavelength of λmax=285 nm, characteristic of α-tocopherol
acetate. The eluent flow rate was 1.0 ml/min, the sample volume tested was 10 μl. The
chromatography temperature was 250C. The analysis duration was 40 minutes.
Results:
To prepare the test solution, 5 ml of the sample is measured into a 100 ml volumetric
flask, 20 ml of isopropanol is added, mixed and the volume of the solution is brought to the mark
with isopropanol. 0.050 g of α-tocaferol acetate is dissolved in isopropanol in a 50 ml volumetric
flask and brought to the mark with this solvent.
According to the results of chromatography, the chromatogram of the standard α-tocopherol
acetate sample showed characteristic absorption lines at 2.764 nm. The characteristic absorption lines
of the ME-1 sample appeared at 2.801 nm, and the ME-2 sample appeared at 2.812 nm. The literature
states that the maximum absorption lines of α-tocopherol acetate appear at 295 nm.
The amount of α-tocopherol acetate in the samples was calculated according to the formula.
According to the results, the amount of α-tocopherol acetate in ME-1 obtained by ultrasonic method
was higher than in ME-2 obtained by simple maceration method.
400
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
It was determined through experiments that the oil extracts obtained from local
medicinal plant raw materials: calami rhizomata, hypericum (Herba hyperici), glycyrrhizae root
(Radices Glicyrrhizae), yarrow (Herba Bidentis) and rosehip fruit (Fructus Rosae) contained 0.43%
vitamin E in ME-1 and 0.51% in ME-2.
