253
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
PHYTOCHEMICAL STUDY OF COUMARINS IN THE AERIAL PARTS OF
PRANGOS FEDTSCHENKOI (REGEL ET SCHMALH.) KOROV
Komilov Kh.M.
Ikramova M.Sh.
Mukhitdinova M.K.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
https://doi.org/10.5281/zenodo.17333654
Background.
Coumarin compounds are derivatives of cis-ortho-hydroxycinnamic acids. They
are known for their biological activities, particularly their anticoagulant effects, while furocoumarins
act as photosensitizers, i.e., they increase skin sensitivity to UV radiation (applied in the treatment of
vitiligo). From this perspective, the search for coumarin-containing plants, isolation of their bioactive
compounds, identification of their chemical structures, and study of their pharmacological activity
are highly relevant.
In the flora of Uzbekistan, the genus Prangos L. is represented by 8 species. The coumarins of
the studied species have been poorly investigated. The aerial parts of Prangos fedtschenkoi grow in
the mountainous regions of the Tian Shan. For the study, the plant was collected in the mountainous
areas of Kashkadarya during late flowering (June), dried in the shade, and crushed.
Objective.
From 1.0 kg of raw material, extraction was carried out three times with 95%
ethanol. The combined extracts were concentrated under vacuum, diluted with water (1:2), and
extracted three times with 100 mL portions of diethyl ether. The ether fractions were combined, dried
over anhydrous Na₂SO₄, and evaporated. The aqueous phase, which was not transferred to ether, was
extracted three times with 100 mL portions of ethyl acetate using a separatory funnel. The ethyl
acetate fraction was collected.
Methods.
The ether fraction was chromatographed in the solvent system n-hexane–benzene–
methanol (5:1:4) (System A) or petroleum ether–benzene–ethanol (5:1:4). The ethyl acetate fraction
was chromatographed in n-butanol–acetic acid–water (5:1:4) (System B). Both chromatograms were
dried, examined under UV light, sprayed with 10% ethanolic KOH, dried at 105 °C, and treated with
freshly prepared diazo reagent. Spots characteristic of coumarins were revealed, and their Rf values
were determined.
In the ether fraction, spots at Rf 0.30, 0.62, 0.70, and 0.86 were observed. These spots appeared
reddish and showed violet-blue fluorescence under UV light.
In the ethyl acetate fraction, a reddish spot with Rf 0.80 was detected (System C: acetic acid–
water, 6:4).
The Rf 0.72 spot from the ether fraction chromatogram corresponded to coumarin, identified
by comparison with the reference compound umbelliferone.
The ethyl acetate fraction was evaporated to dryness, and the residue was hydrolyzed by adding
10% sulfuric acid and refluxing with a condenser in a water bath for 5 hours. After cooling, the
hydrolysate was extracted three times with 20 mL portions of diethyl ether.
Paper chromatography of the ether-soluble portion revealed a spot at Rf 0.72, which matched
umbelliferone as the reference compound.
The water-soluble portion remaining after hydrolysis gave a spot at Rf 0.77, identified as a
carbohydrate. Using paper chromatography (System C) and spraying with aniline phthalate reagent,
it was identified as D-glucose.
254
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Results and Conclusion.
From the ether and ethyl acetate fractions of Prangos fedtschenkoi,
the aglycone umbelliferone and D-glucose were identified after hydrolysis. Based on the Rf 0.72 spot
corresponding to umbelliferone and the Rf 0.80 spot corresponding to umbelliferone glycoside, the
compound present in the aerial parts of the plant was determined to be skimmin (umbelliferone-7-O-
β-D-glucoside).
