European International Journal of Pedagogics
231
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TYPE
Original Research
PAGE NO.
231-234
DOI
3
OPEN ACCESS
SUBMITED
31 March 2025
ACCEPTED
29 April 2025
PUBLISHED
31 May 2025
VOLUME
Vol.05 Issue05 2025
COPYRIGHT
© 2025 Original content from this work may be used under the terms
of the creative commons attributes 4.0 License.
Positive Effect of
Pseudomonas
Chlororaphis On Mung
Bean Germination
M. Qalandarova
Teacher of Nizami State Polytechnic University, Uzbekistan
T. Usanov
Senior Research Fellow of the Scientific Research Institute of
Microbiology, Uzbekistan
R. Norboboyeva
Senior Teacher of Nizami State Polytechnic University, Uzbekistan
Abstract:
In this article, we can see that the bacterium
Pseudomonas chlororaphis had a very positive effect on
germination when exposed to two different varieties of
mung bean plants using two different dilutions of this
strain. We observed that the most effective results were
obtained from the dilutions of the bacterial strain at
1:19 and 1:49, with the 1:19 dilution being the most
effective.
Keywords:
Fusarium solani, Aspergillus ochraceus,
Fusarium culmorum, Pseudomonas chlororaphis,
Cladosporium sp., Aspergillus flavus, Fusarium
verticillioides, Alternaria tenuissima, Navro'z, Kahrabo,
Inhibition zone, phytopathogenic fungi.
Introduction:
Phytopathogenic fungi cause significant
damage to plants. Phytopathogenic fungi are a serious
problem in agriculture, negatively affecting crop quality
and productivity [1]. The activity of phytopathogenic
fungi in plants is difficult to control, causing significant
losses for many crops [2]. Bacterial strains that combat
such phytopathogenic fungi and stimulate plant growth
are very useful. On this basis, we aimed to observe the
positive effect of the bacterium Pseudomonas
chlororaphis on the vegetative period of mung bean
plants and the high antagonistic activity of this bacterial
strain against phytopathogenic fungi such as Fusarium
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European International Journal of Pedagogics
solani,
Aspergillus
ochraceus,
and
Fusarium
culmorum[3].
The research used a strain of the bacterium
Pseudomonas chlororaphis from the collection of the
Microbiology Laboratory and phytopathogenic fungi
such as Fusarium solani, Aspergillus ochraceus, and
Fusarium culmorum. Two widely cultivated varieties of
mung bean, Navruz and Qahrab, were used in
agriculture. In laboratory conditions, phytopathogenic
fungi and bacterial strains were grown on Mpa and
Chapek nutrient media. Antagonistic activity was
assessed using the well-diffusion method. Agar
medium was placed in Petri dishes, phytopathogenic
fungi were sown using the lawn method, and bacterial
culture fluid was placed in the well. Incubation was
continued for 3-7 days at 30°C, and the affected zones
were measured in millimeters, and the bacterial strain
showed a high level of antagonistic activity against these
phytopathogens. When seeds from two different
varieties of mung bean were exposed to a liquid-based
method, germination was significantly affected
compared to the control. Two different varieties of this
mung bean plant are widely cultivated in our country.
This had a positive effect on both varieties, and it was
especially effective on the Navruz variety. When seeds
were treated with a liquefied solution of this bacterial
strain, we can see that the 1:19 ratio produced the most
effective results when the liquefied solution was
prepared in two different ratios, 1:19 and 1:49. We can
see that there was a significant difference in the
germination of mung bean plants when treated with a
dilution of the bacterial strain compared to the control.
(figure 2.)
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European International Journal of Pedagogics
Figure 2. Effect of bacterial strain on mung bean germination
We can see that the germination of the mung bean
plant was positively affected by the dilution of the
bacterial solution at a ratio of 1:19. When seed
germination was monitored compared to the control,
we observed that 1:19 and 1:49 were also very
effective. According to the results, the P. chlororaphis
strain formed inhibition zones of 28
–
34 mm against
Fusarium solani, Aspergillus ochraceus, and Fusarium
culmorum. It had moderate antagonistic activity against
Penicillium sp., Fusarium oxysporium, and Alternaria
alternata, and no significant antagonistic activity was
observed against Cladosporium sp., Aspergillus flavus,
Fusarium verticillioides, and Alternaria tenuissima.
Figure 1. Effect of Pseudomonas chlororaphis on phytopathogenic fungi
Phytopathogenic fungi
Diameter of the inhibition zone, mm
Fusarium solani
34
Aspergillus orchaceus
29
Fusarium culmorium
28
Penicillium sp.
15
Fusarium oxysporium
14
Alternaria alternata
13
Cladosporium sp.
-
Aspergillus flavus
-
Fusarium verticillioides
-
Alternaria tenuissima
-
table 1.
The result observed when the zone of inhibition of
phytopathogenic fungi under the influence of the
bacterium Pseudomonas chlororaphis was measured
in diametric mm. In an experiment conducted on the
Navruz and Qahrab varieties of mung bean, dilutions of
P. chlororaphis strain at 1:19 and 1:49 had a positive
effect on seed germination. In particular, the Navruz
variety produced 14
–
19 seeds at a ratio of 1:19, which
was a significant result. The highest result (12) was
recorded in the Kahraba variety at a ratio of 1:49. In
conclusion, the study results showed that the
Pseudomonas chlororaphis strain has high antagonistic
activity against some phytopathogenic fungi. When
applied to mung bean plants, seed germination rates
increased. This bacterium is considered promising as a
biological control agent and as a biofertilizer.
REFERENCES
Nizametdinova Ya.F., Mansurova M.L., Muzaffarova I.A.,
Kondrateva K.V., Vakhobov A.H. Practical exercises in
microbiology. Tashkent, Tashkent State University,
1992.
Burkhonova H.K., Murodov M.M. Microbiology.
European International Journal of Pedagogics
234
https://eipublication.com/index.php/eijp
European International Journal of Pedagogics
Tashkent, Teacher, 1975.
Synergistic antifungal mechanism of cinnamaldehyde
and nonanal against Aspergillus flavus. Food
Microbiology, 104524 (2024). Selin
C., Habibian R., Poritsanos N., Athukorala S., Fernando
D., de Kievit T. “Phenazines are not essential for
Pseudomonas chlororaphis PA23 biocontrol of
Sclerotinia sclerotiorum, but do play a role in biofilm
formation.” FEMS Microbiology Ecology. 2010. Volume
71:1. p. 73-83.
Palleroni N. Genus I. Pseudomonas. In Bergey’s Manual
of Systematic Bacteriology, 2nd edition. 2005. Volume
2. p. 323-379
Bloemberg G., Lugtenberg B .
“Molecular basis of plant
growth promotion and biocontrol by rhizobacteria.”
Current Opinion in Plant Biology. 2001. Volume 4: 4.
p.343-350
