130
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF QUALITY PARAMETERS OF A LIQUID EXTRACT BASED
ON LOCAL MEDICINAL PLANTS USED IN INFLAMMATORY DISEASES OF THE
GASTROINTESTINAL TRACT
Ulug'bekova S.T.
1
Pazilbekova Z.T.
2
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: Sitoraxayrullayeva77@gmail.com
https://doi.org/10.5281/zenodo.17325175
Relevance:
Peptic ulcer disease of the stomach and duodenum is a chronic, recurrent disorder
characterized by mucosal defects and ulcer formation in the stomach and duodenal walls. According
to statistical data, 10–12% of adults, mainly between 20 and 50 years of age, suffer from
gastroduodenal ulcers. The production volume of drugs used in the treatment of gastrointestinal
inflammatory diseases does not meet existing demand, and most of these medications are imported.
Since gastrointestinal inflammatory diseases require long-term therapy, the prolonged use of
synthetic drugs often leads to adverse effects, accumulation in the div, and toxic effects on other
organs. This situation has increased the demand for dosage forms based on medicinal plants. In the
State Register of the Republic of Uzbekistan, Chamomile (Matricaria chamomilla), Calendula
(Calendula officinalis), and St. John’s wort (Hypericum perforatum) have been studied as medicinal
plants, and pharmacopoeial monographs have been approved for them. However, no finished dosage
forms combining these plants have yet been developed. Therefore, the development of a complex
herbal mixture and its corresponding liquid extract for the treatment of gastrointestinal inflammatory
diseases, as well as the evaluation of its quality, is of significant importance.
Objective.
To develop a complex herbal mixture and evaluate the quality of a new liquid extract
with anti-inflammatory, analgesic, and antiseptic properties for use in gastrointestinal inflammatory
diseases.
Materials and methods.
A liquid extract was obtained from local medicinal raw materials—
Chamomile flowers (Matricaria chamomilla), Calendula flowers (Calendula officinalis), and the
aerial part of St. John’s wort (Hypericum perforatum)—using the maceration method. Ethanol at
concentrations of 40% and 70% was used as the extraction solvent. The liquid extracts were evaluated
for quality parameters, including organoleptic properties (appearance, odor, taste, color), dry residue,
ethanol content, authenticity, heavy metal content, and active constituents, in accordance with the
requirements of the State Pharmacopoeia of the Republic of Uzbekistan, 1st edition (UzR Ph I), and
other normative documents.
Results.
The liquid extracts obtained from the complex herbal mixture were clear, yellow-
brown liquids with a characteristic odor and slightly bitter taste. The main active constituents were
flavonoids, which provide anti-inflammatory and antiseptic activity. The extracts were evaluated for
appearance, odor, taste, color, dry residue, ethanol concentration, heavy metal content, and active
substances. The ethanol content was found to be 33% in the extract obtained with 40% ethanol, and
66% in the extract obtained with 70% ethanol. Heavy metal content was below the permissible limit
0.01%. The dry residue ranged between 5.9–6.8%, corresponding to pharmacopoeial requirements.
The flavonoid
content was determined spectrophotometrically and is under further investigation.
Conclusion.
The liquid extracts obtained by maceration using 40% and 70% ethanol from a
complex herbal mixture of Chamomile (Matricaria chamomilla), Calendula (Calendula officinalis),
and St. John’s wort (Hypericum perforatum) meet the quality requirements of the State
131
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Pharmacopoeia of the Republic of Uzbekistan (1st edition) in terms of both qualitative and
quantitative parameters. The developed extract is recommended as a new dosage form for the
treatment of gastrointestinal inflammatory diseases.
