524
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
UNDER X-RAY IRRADIATION
Rzayeva I.A.
Valieva M.N.
Aliyev N.A.
Musayeva S.E.
Yagubova S.G.
Atakishizade S.A.
Azerbaijan Medical University, Baku, Republic of Azerbaijan
ilaheadil@gmail.com tel:
23
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72
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222
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50
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+994
https://doi.org/10.5281/zenodo.17343910
Relevance:
It has been shown that the effect of X-ray irradiation at 2 Gy and 4 Gy leads to
suppression of the activity of the studied enzymes in various brain structures. Moreover, it was found
that pre-administration of saffron extract in many cases prevents significant suppression of the studied
enzymes by irradiation, and on the contrary, this antioxidant promotes an increase in their activity in
brain structures. Objective: To study the effect of saffron (Crocus sativus L.) stigma extract on the
dynamics of changes in the content of different types of surface-exposed SH-groups, structurally
masked protein SH-groups, and glutathione under X-ray irradiation at doses of 2 and 4 Gy in different
brain regions.
Materials and Methods:
The study was conducted on male rats weighing 180 ± 20 g. The experimental design was as
follows:
•
Group I – control
•
Group II – X-ray irradiation
•
Group III – saffron extract + X-ray irradiation
For 21 days prior to irradiation, animals received saffron extract per os at a dose of 120 mg/kg.
Measurements were taken 1 hour, 3 days, and 6 days after irradiation. X-ray irradiation was
performed using the “RUM-17” device at doses of 2 Gy and 4 Gy. The content of various SH-groups
in homogenates was determined according to Ellman G.L. as modified by Sedlak J. and Lindsay R.N.
(1968) [2, 3]. The method is based on the reaction of 5,5’-dithiobis(2-nitrobenzoic acid) with aliphatic
thiol compounds at pH 8.0, forming 1 mole of 2-nitro-5-mercaptobenzoic acid per mole of thiol. The
anion of this acid has an intense yellow color, which is used for spectrophotometric determination of
thiol compound concentrations
Results:
Data were processed by Student’s t-test. Results showed that X-ray irradiation leads
to a decrease in thiol group levels in the div. The antioxidant system is unable to fully compensate
for the effects of radiation exposure, therefore external radioprotectors are required.
Conclusions:
Thus, in the studied brain structures, X-ray irradiation (4 Gy) caused a reduction
in thiol group content depending on the observation time. Administration of saffron extract stabilizes
thiol group concentrations and protects against the oxidative effects of free radical compounds, which
are intensified by irradiation.
