394
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
MACRO- AND MICROELEMENTS IN THE AERIAL PARTS OF
ATRAPHAXIS
PYRIFOLIA
A. Yulchiyeva
PhD Student (Basic Doctoral Student), Namangan State University
Sh.V. Abdullayev
Doctor of Chemical Sciences (DSc), Professor, Namangan State University
N.G. Abdullajonova
Leading Researcher, Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan,
Doctor of Chemical Sciences (DSc), Professor
https://doi.org/10.5281/zenodo.17339569
In our research, the main objective was defined as the study of the chemical components of
Atraphaxis pyrifolia
, a plant belonging to the Polygonaceae family.
In June 2025, we initiated the qualitative and quantitative analysis of macro- and microelements
in the aerial parts of
Atraphaxis pyrifolia
, collected in the mountainous areas of Parkent District,
Tashkent Region, as part of the study of its chemical components.
The analysis of the chemical composition of the studied plant provides an opportunity to draw
more precise conclusions about this species.
An accurately weighed sample of 0.1000 g was quantitatively transferred into Teflon
autoclaves. Subsequently, 3 mL of purified concentrated nitric acid (HNO₃) and 2 mL of purified
hydrogen peroxide (H₂O₂) were added. The autoclaves were sealed and placed into a microwave
digestion system Berghof (SpeedWave Xpert or a similar microwave oven). The digestion process
was carried out according to a specific program set via the instrument interface. The number of
autoclaves, as well as the temperature and pressure inside them, were automatically controlled and
monitored by the system. Process parameters were displayed on a liquid-crystal display. The digestion
was conducted under conditions of moist decomposition, with a minimum temperature of T = 50 °C
and a maximum temperature of T = 230 °C, at a maximum pressure of P = 40 bar, for a duration of
35–45 minutes. After digestion, the autoclaves were cooled to room temperature, and the liquid
mixture was quantitatively transferred to a volumetric flask of 50 or 100 mL capacity. Each autoclave
was rinsed 2–3 times, and the rinsings were combined, after which the flask was filled to the
calibration mark with distilled water.
The solution was thoroughly mixed, transferred into an autosampler vial, and placed in the
designated position of the autosampler. In the program, the position of each vial, the weighed mass,
and the dilution factor were entered.
The mineralized solution was analyzed using an Avio-200 ICP-OES (Inductively Coupled
Plasma Optical Emission Spectrometer, PerkinElmer) to quantitatively determine the macro- and
microelements, heavy metal salts, and rare metals relative to the corresponding standard samples. At
the end of the process, the instrument automatically recalculated the results based on the sample mass
and dilution factors, providing the degree of accuracy and the relative standard deviation (RSD)
values.
Table 1 presents the chemical elemental composition of the aerial parts of
Atraphaxis pyrifolia
.
Table 1
Determination of Macro- and Microelement Contents in the Samples by the Avio 200
(ICP-OES) Optical Emission Spectrometric Method
Elements
Stem
Flower
Leaf
395
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
1
2
3
4
Al
1,125
1.265
2.437
Ba
0.750
1.248
2.647
B
0.086
0.101
0.102
V
0.064
0.045
0.035
Bi
0
0
0
Fe
1.288
3.744
1.878
Cd
0.033
0.022
0.024
Sr
0.212
0.273
0.608
K*
45.063
126.408
102.265
Ca*
80.440
144.507
536.259
Co
0.033
0.032
0.033
Si
0.434
1.572
0.732
Li
0.067
0.089
0.732
Mg*
3.313
7.582
15.068
Mn
0.182
0.525
2.344
Cu
0.318
0.210
0.140
As
0
0.007
0
Na*
2.388
17.608
4.659
Ni
0.094
0.172
0.132
Pb
0.019
0.001
0
Se
0.216
0.186
0.201
Ag
0
0
0
Sn
0
0
0
Ti
0.083
0.046
0.066
Cr
0.052
0.054
0.052
Zn
0.332
0.424
0.316
P*
4.637
15.152
12.251
Mo
0.014
0.023
0.013
Hg
0
0
0
S
2.384
2.466
2.899
The analysis of the macro- and microelements in the aerial parts of
Atraphaxis pyrifolia
revealed that elements such as potassium (K), calcium (Ca), and magnesium (Mg) were present in
higher amounts in the flowers, stems, and leaves of the plant. In addition, aluminum (Al), iron (Fe),
sulfur (S), phosphorus (P), and sodium (Na) were also detected in comparatively higher amounts than
other elements, although at lower concentrations overall. Importantly, toxic elements such as Pb, Cd,
As, and Hg were not detected in the composition of
Atraphaxis pyrifolia
, and the levels of other heavy
metals did not exceed permissible limits. On the contrary, the relatively high content of magnesium,
potassium, and calcium in different plant organs highlights the significance of the studied plant as a
subject of research.
