348
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
PHYTOCHEMICAL STUDY OF
PEROVSKIA SCROPHULARIIFOLIA
Atqiyaeva S.I.
Okhundedaev B.S.
Botirov E.Kh.
Sasmakov S.A.
S.Yu. Yunusov Institute of Plant Chemistry, Tashkent city, Republic of Uzbekistan
e-mail: atqiyayevasanobarxon1992@gmail.com
https://doi.org/10.5281/zenodo.17337420
Relevance
: A decoction of the above-ground part of
Perovskia scrophulariifolia
Bunge (family
Lamiacaeae) is used in folk medicine for scabies in humans and animals, as well as for healing
sunburn and skin diseases. Dried flowers of the plant are brewed and consumed as tea to relieve
gastrointestinal diseases and abdominal pain. In some countries of the Asian region,
P.
scrophulariifоlia
is used to remove intestinal parasites from the div. The above-ground part of
P.
scrophulariifolia
contains
essential
oil,
abietane
diterpenoids,
flavonoid
diosmetin,
phenylpropanoids, anthocyanins, nitrogenous compounds, eugenol glycosides, benzyl alcohol,
salidroside and terpenoids. The chemical composition of the roots of
P.scrophulariifolia
has not been
studied.
Purpose of the study
. Isolation and study of biologically active components of the
aboveground part and roots of
P. scrophulariifolia
collected in the vicinity of the village of
Omonkuton, Samarkand region of the Republic of Uzbekistan.
Materials and methods.
The crushed air-dried plant material (aerial part and roots) was
extracted five times at room temperature with methanol. The combined extract was evaporated in a
vacuum, diluted with water and subjected to liquid-liquid extraction with gasoline, chloroform, ethyl
acetate and n-butanol.
Results.
Four individual substances (1-4) were isolated from different fractions of the methanol
extract of the above-ground part by column chromatography on silica gel and Sephadex LH-20, and
seven compounds (5-11) were isolated from the roots. Based on the study of UV, 1H and 13C NMR
spectra and the X-ray diffraction method, the isolated substances were identified with carnosol (1),
cirsimaritin (2), stigmasterol (3) and β-sitosterol (4), cryptotanshinone (5), tanshinone IIA (6),
przewalskin Y-1 (7), grandifolia B (8), rosmarinic (9), caffeic acids (10) and D-pinitol (11). Carnosol
has antioxidant, antitumor and anti-inflammatory properties. D-Pinitol has antidiabetic and
antioxidant activity, it is recommended for the treatment of conditions associated with insulin
resistance, such as diabetes mellitus and its chronic complications.
To study the antibacterial and antifungal properties of the benzene, chloroform and ethyl acetate
fractions of the aboveground part of
P. scrophulariifolia
, a modified agar diffusion method was used
using the following microorganism strains as test cultures: gram-positive bacteria -
Bacillus subtilis
(RKMUz - 5),
Staphylococcus aureus
(ATCC 25923); gram-negative bacteria -
Pseudomonas
aeruginosa
(ATCC 27879),
Escherichia coli
(RKMUz - 221) and the fungal strain
Candida albicans
(RKMUz - 247). Impregnated disks with ampicillin/sulbactam, gentamicin and fluconazole
(Himedia) were used as positive, and extraction solvents as negative controls.
The test results showed that the gasoline fraction has a pronounced antibacterial effect on the
bacterial strains
B. subtilis
(12.08±0.12 mm),
S. aureus
(11.04±0.10 mm) and
P. aeruginosa
(11.04±0.10 mm). The chloroform and ethyl acetate fractions were inactive against the studied
bacterial and fungal strains.
All the described substances were isolated from
Рerovskia scrophulariifolia
for the first time.
349
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions.
It has been shown that the plant
P. scrophulariifolia
is a rich source of
biologically active diterpenoids, flavonoids, phenolic carboxylic acids, phytosterols and can be used
to create effective medicines and dietary supplements.
