305
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
QUALITY CONTROL AND STANDARDIZATION OF BIOACTIVE COMPOUNDS
IN THE DRY EXTRACT 'EXTRADENT'
Yuldasheva Sh.S.
Akhmadjonova D.A.
'Alfraganus University, Tashkent city, Republic of Uzbekistan
Andijan State Medical Institute, Andijan city, Republic of Uzbekistan
e-mail:
https://doi.org/10.5281/zenodo.17335244
Relevance:
Herbal preparations remain an important direction in pharmaceutical science due
to their wide pharmacological effects and relatively low toxicity. 'Extradent' is a dry extract consisting
of Calendula officinalis, Urtica dioica, Capsella bursa-pastoris, and Polygonum hydropiper. These
plants are known to contain flavonoids, carotenoids, tannins, amino acids, and mineral elements that
contribute to the therapeutic properties of the preparation.
Keywords:
Extradent, quality control, standardization, rutin, flavonoids, HPLC, UV-
spectrophotometry, carotenoids, tannins, amino acids, minerals.
Purpose of the research.
To ensure quality control and standardization of the dry extract
'Extradent' by identifying its main biologically active substances, with special attention to flavonoids.
Methods.
High-performance liquid chromatography (HPLC) and UV-spectrophotometry were
applied. The extract was dissolved in 70% ethanol and filtered. Chromatographic analysis was carried
out on a C18 column with acetonitrile, water, and acetic acid as the mobile phase. UV-
spectrophotometry was used to measure the total flavonoid content using the AlCl₃ complex method.
Results .
The chromatogram revealed several phenolic compounds, with rutin identified as the
main marker. Its clear peak confirmed its suitability for standardization. Spectrophotometric analysis
showed a significant presence of flavonoids beyond rutin, indicating the complex composition of the
extract. Literature data confirm that carotenoids, tannins, amino acids, and minerals also strengthen
the therapeutic potential of 'Extradent'.
Conclusion.
The combination of HPLC and UV-spectrophotometry is an effective approach
for the quality control of 'Extradent'. Rutin can be used as a reliable marker, while the presence of
other biologically active compounds ensures a wide spectrum of pharmacological activity. The results
provide a basis for certification and broader medical application of the preparation.
