271
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
NEUTRAL LIPIDS OF THE AERIAL PART OF
ARTEMISIA JUNCEA
Samandarova Z.Y.
Yuldasheva N.K.
Gusakova S.D.
Nishanbayev S.Z.
S.Y. Yunusov Institute of the Chemistry of Plant Substances, Tashkent city, Republic of
Uzbekistan
e-mail: zarifasamandarova1005@gmail.com
https://doi.org/10.5281/zenodo.17334162
Introduction.
Artemisia juncea
Kar. et Kir. (rush-like wormwood, family Asteraceae) is a
perennial herbaceous halophytic plant growing on moderately saline soils and is one of the most
widespread Artemisia species in the flora of Uzbekistan.
A. juncea
is widely used in folk and
traditional medicine. The leaves and stems are used in the treatment of liver diseases, the
inflorescences are applied for rhinitis, cough, and fever, and pastes made from the fresh plant are used
externally for abscesses and acne. The aerial part and roots of the plant are used in Tibetan medicine
for the treatment of tumors, abscesses, as an anthelmintic, and for anthrax.
The aim
of this study was to investigate the neutral lipids of the aerial part of
A. juncea
collected
in the Bukhara region.
Materials and methods.
The content of neutral lipids (NL) was determined in triplicate by
maceration using extraction-grade petroleum ether (b.p. 72–80 °C). The qualitative composition of
NL classes was identified by analytical thin-layer chromatography on silica gel using a hexane–
diethyl ether (4:1) solvent system. The identification of spots on the plates was carried out based on
specific reactions and by comparing the (R
f
) values with those of reference compounds.
Results.
The content of NL was 1,60% on a dry weight basis. The NL composition included
unsaturated hydrocarbons, aliphatic alcohols, free fatty acids, free triterpenols, and phytosterols.
Triacylglycerols were present in trace amounts. According to spectrophotometric data, the carotenoid
content in NL was 25,0 mg%. Fatty acids were isolated from NL by alkaline hydrolysis, converted to
their methyl esters using diazomethane, and analyzed by gas chromatography on an Agilent 8860GC
(table).
Table. Composition of fatty acids of neutral lipids of the aerial part of A. juncea, GC, %
of acids mass
Conclusions.
The results obtained show that the aerial part of
A. juncea
growing in Uzbekistan
contains 1,60% NL. The fatty acid composition consists of 16 components, with unsaturated fatty
acids predominating (64,09%). The full set of high-molecular-weight fatty acids 20:0, 22:0, and 24:0
was identified.
Acid
%
Acid
%
10:0, 14:0, 15:0, 17:0
8,10
20:0
1,45
16:0
20,35
20:1
1,29
16:1
3,97
22:0
1,97
18:0
2,84
22:1
2,37
cis-
18:1n9
13,98
24:0
1,20
trans
- 18:1
3,93
24:1
1,69
18:2n6
30,31
Σsaturated fatty acid
35,91
18:3n3
6,55
Σunsaturated fatty acid
64,09
272
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Financing.
This work was carried out using budgetary funds of ICPS the Academy of Sciences
of the Republic of Uzbekistan. We thank the Academy of Sciences of the Republic of Uzbekistan for
supporting this study.
