ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
47
STUDYING THE MORPHOMETRIC INDICATORS OF THE PANCREAS
IN LABORATORY ANIMALS WITH INDUCED HYPERTIRHOSIS.
Togaeva G.S.
Samarkand State Medical University, Uzbekistan
Oripov F.S.
Scientific supervisor: Doctor of Medical Sciences, Professor
https://doi.org/10.5281/zenodo.16741604
Annotation.
Thyroid dysfunction is polyetiological and may be associated
with developmental anomalies, genetic pathology, T-suppressor deficiency, and
inflammatory process in thyroid tissue. The study used 30 rats to study the
effects of experimental thyrotoxicosis. The rats were divided into two groups -
the group with intact animals and the group with experimental
hyperthyroidism. Hyperthyroidism was modeled by administering L-thyroxine
in various doses over 14 days. Comparative analysis of the morphometric
indicators of pancreatic pancreatic cells in control and experimental rats
showed that despite the fact that the total cell volume, cytoplasm volume, and
nuclear volume of pancreatic cells in hyperthyroid rats were lower than in the
control group animals.
Keywords:
endocrine system, thyroid gland, pancreas, experimental
animals, hyperthyroidism.
Relevance.Currently, there is a steady increase in thyroid pathology,
therefore, the development of experimental models is of great importance for
studying the pathogenesis, clinical presentation, treatment, and prevention of
these diseases, the selection of which requires consideration of adequacy,
validity, bioethics, and sensitivity to various physiological, pharmacological,
genetic, and other manipulations. The World Health Organization notes that
with each passing year, thyroid diseases are detected more frequently,
approximately in every tenth inhabitant of the planet [1, 3, 5]. Thyroid
dysfunction is polyetiological and can be associated with developmental
anomaly, genetic pathology, T-suppressor deficiency, inflammatory process in
thyroid tissue, congenital defect of enzyme systems, drug therapy, strumogenic
effect of microelement deficiency, and other causes [2]. Taking into account the
fact that the incidence of thyroid disease has been increasing recently,
researchers' interest is directed towards the formation (selection) of
experimental models of endocrine diseases for the purpose of studying and
analyzing the pharmacological properties of new compounds, as well as reliably
identifying the peculiarities in the mechanism of action of already known drugs
[4].
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
48
Work objective.
Assess the results of a comparative analysis of the
morphological and morphometric indicators of the pancreatic parenchyma
resulting from experimental hyperthyroidism.
Materials and methods.
The study used 30 rats to study the effects of
experimental thyrotoxicosis. The rats were divided into two groups - the group
with intact animals and the group with experimental hyperthyroidism.
Hyperthyroidism was modeled by administering L-thyroxine in various doses
over 14 days. The material taken from the experimental animals after
decapitation under anesthesia was placed in a plastic container for fixation in a
10% solution of neutralized formalin. Formalin cuts were washed with running
water, dehydrated in increasingly concentrated alcohols, and paraffin blocks
were prepared. Histological sections 5-7 μm thick were made from paraffin
blocks and examined by hematoxylin-eosin staining and Van Gieson staining.
Morphometric measurements were carried out at the resolution of the
microscope 10×10, 10×20, and 10×40. Statistical processing of morphometric
indicators was carried out using the Student-Fisher criterion, using the standard
error of the arithmetic mean M/m of the relative value and the significance
coefficient of the t-difference. As a result of the research, certain results were
obtained.
Research results.
For the experiment, 30 rats of the same age with the
same div weight were selected. Morphometric indicators were studied. By
creating an experimental model of hyperthyroidism, the parameters of
morphological and morphometric reactive changes in the interstitial tissue of
the pancreas and pancreatic cells of rats were studied and compared.
Comparative morphometric indicators of pancreatic pancreatic cells in
rats (in relative units)
Table No. 1
Nuclear
volume
Cytoplasm
volume
Total
volume
Oy/Os ratio
Control group
3.95±0.11
11.42±0.33
15.37±0.40 0.36±0.01
Experimental
hyperthyroidism
3.02±0.32
7.44 ± 0.29
10.46 ± 0.34 0.43 ± 0.02
Conclusion:
Comparative analysis of the morphometric indicators of
pancreatic pancreatic cells in control and experimental rats showed that despite
the fact that the total cell volume, cytoplasm volume, and nuclear volume of
pancreatic cells in hyperthyroid rats were smaller than in the control group
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
49
animals, the nuclear-cytoplasmic ratio was significantly higher by 15%. This
situation means that the proliferative reaction of these cells in response to
experimental exposure increases, which in turn can affect the organ's functional
state.
References:
1. Lavrinenko A. M., Botasheva M. M. Nephropathy in rats with experimental
hyperthyroidism. International Journal of Applied and Basic Research, 2015, no.
5-1,
pp.
62-63.
Available
at:
https://applied-
research.ru/ru/article/view?id=6762 (accessed 13 November 2019).
2. Litvitsky P. F. Pathology of the endocrine system. Etiology and pathogenesis of
endocrinopathies: dysfunctions of the thyroid and parathyroid glands/Questions
of modern pediatrics, 2012, vol. 11, no. 1, pp. 61-75.
3. Lobyreva O. V. Thyroid status and its effect on the activity of oxidative
enzymes. Scientific notes of the Kazan State Academy of Veterinary Medicine
named after N.E. Baumana, 2010, vol. 201, pp. 259-263.
4. Mayanskaya N. N., Rymar S. S., Mayanskaya S. Features of the inflammatory
process in rats with experimental hypo- and hyperthyroidism. Kazan Medical
Journal, 2013, vol. 94, no. 5, pp. 726-730.
5. Miroshnikov S. V., Notova S. V., Timasheva A. B., Kvan O. V. The effect of
experimental thyrotoxicosis and hypothyroidism on the elemental status of
laboratory animals. Modern Problems of Science and Education, 2013, No. 3.
Available at: http://science-education.ru/ru/article/view?id=9336 (accessed 13
November 2019)
